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Types of Bacterial Stocking / Preservation

Types of Bacterial Stocking / Preservation Bacterial stocking aims to store bacteria that are still viable for future use. There are 2 types of stocking: 1. Short Term Keeping    Short Term Keeping is done routinely, usually once a month, into new media. The procedure for Short Term Keeping is: prepare the required tools and materials, UV LAF, and make new media. Once the media is prepared, inoculate the bacteria onto the media in a slant tube and store it in a fridge at 2-8°C. 2. Long Term Keeping Long Term Keeping requires a cryoprotectant agent to protect bacterial cells during storage in a deep freezer at -80°C to prevent cell damage at extreme temperatures. The cryoprotectant agent used for long-term storage is glycerol.  The procedure for this method is: prepare the required tools and materials, UV LAF, and make Broth media. The isolate to be stocked is inoculated into 5-7 mL of liquid media in a slant tube. The bacterial inoculum is then shaken for 24 hours. After ...
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Types of Bacterial Stocking / Preservation

Types of Bacterial Stocking / Preservation


Bacterial stocking aims to store bacteria that are still viable for future use. There are 2 types of stocking:

1. Short Term Keeping  

Short Term Keeping is done routinely, usually once a month, into new media. The procedure for Short Term Keeping is: prepare the required tools and materials, UV LAF, and make new media. Once the media is prepared, inoculate the bacteria onto the media in a slant tube and store it in a fridge at 2-8°C.

2. Long Term Keeping

Long Term Keeping requires a cryoprotectant agent to protect bacterial cells during storage in a deep freezer at -80°C to prevent cell damage at extreme temperatures. The cryoprotectant agent used for long-term storage is glycerol. 

The procedure for this method is: prepare the required tools and materials, UV LAF, and make Broth media. The isolate to be stocked is inoculated into 5-7 mL of liquid media in a slant tube. The bacterial inoculum is then shaken for 24 hours. After that, take 1 mL of the culture and add it to sterile glycerol using a 10 mL micropipette, then vortex to homogenize. The final step is to store it in a deep freezer at -80°C.

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