Types of Bacterial Stocking / Preservation Bacterial stocking aims to store bacteria that are still viable for future use. There are 2 types of stocking: 1. Short Term Keeping Short Term Keeping is done routinely, usually once a month, into new media. The procedure for Short Term Keeping is: prepare the required tools and materials, UV LAF, and make new media. Once the media is prepared, inoculate the bacteria onto the media in a slant tube and store it in a fridge at 2-8°C. 2. Long Term Keeping Long Term Keeping requires a cryoprotectant agent to protect bacterial cells during storage in a deep freezer at -80°C to prevent cell damage at extreme temperatures. The cryoprotectant agent used for long-term storage is glycerol. The procedure for this method is: prepare the required tools and materials, UV LAF, and make Broth media. The isolate to be stocked is inoculated into 5-7 mL of liquid media in a slant tube. The bacterial inoculum is then shaken for 24 hours. After ...
Escherichia coli is a pathogenic bacterium that lives in the intestines of humans and animals and plays a role in maintaining a healthy digestive system. This bacterium is also found in water and food, as are non-pathogenic strains of E. coli. Pathogenic strains, such as enterotoxigenic E. coli (EPEC), produce shiga toxin, which is an indicator of water pollution and invades the epithelial cells of the small intestine. The presence of E. coli in aquatic environments indicates contamination by waste or animal feces carried in the water.Escherichia coli is a type of bacteria belonging to the coliform family that normally lives in human and animal feces, therefore it is also called fecal coliform. E. coli is a Gram-negative, rod-shaped, non-spore-forming bacterium. E. coli is commonly found in water as a source of microbial pollution. This bacterium forms rods in chains with a length of approximately 2 micrometers and a diameter of 0.5 micrometers. This bacterium has Bacillus forms includ...