Types of Bacterial Stocking / Preservation Bacterial stocking aims to store bacteria that are still viable for future use. There are 2 types of stocking: 1. Short Term Keeping Short Term Keeping is done routinely, usually once a month, into new media. The procedure for Short Term Keeping is: prepare the required tools and materials, UV LAF, and make new media. Once the media is prepared, inoculate the bacteria onto the media in a slant tube and store it in a fridge at 2-8°C. 2. Long Term Keeping Long Term Keeping requires a cryoprotectant agent to protect bacterial cells during storage in a deep freezer at -80°C to prevent cell damage at extreme temperatures. The cryoprotectant agent used for long-term storage is glycerol. The procedure for this method is: prepare the required tools and materials, UV LAF, and make Broth media. The isolate to be stocked is inoculated into 5-7 mL of liquid media in a slant tube. The bacterial inoculum is then shaken for 24 hours. After ...
Indonesia has rich marine biodiversity and potential for development. Abundant marine resources are not only a food source but can also be developed into medicinal ingredients. Marine invertebrates are the marine organisms that produce the largest number of bioactive compounds compared to marine vertebrates. This is because marine invertebrates have more limited physical movement than marine vertebrates, resulting in a greater response to bioactive compound production. Marine invertebrates with biological activity include sponges, ascidians, mollusks, and bryozoans. In the past decade, the world's attention in medicine has begun to turn to marine organisms as a potential source of these compounds. The need for new antibiotics is high, especially those that are effective in inhibiting resistant pathogenic bacteria. Therefore, information about active antibacterial compounds in the health sector is crucial in combating diseases caused by bacteria. Infectious diseases are diseases cau...