Types of Bacterial Stocking / Preservation Bacterial stocking aims to store bacteria that are still viable for future use. There are 2 types of stocking: 1. Short Term Keeping Short Term Keeping is done routinely, usually once a month, into new media. The procedure for Short Term Keeping is: prepare the required tools and materials, UV LAF, and make new media. Once the media is prepared, inoculate the bacteria onto the media in a slant tube and store it in a fridge at 2-8°C. 2. Long Term Keeping Long Term Keeping requires a cryoprotectant agent to protect bacterial cells during storage in a deep freezer at -80°C to prevent cell damage at extreme temperatures. The cryoprotectant agent used for long-term storage is glycerol. The procedure for this method is: prepare the required tools and materials, UV LAF, and make Broth media. The isolate to be stocked is inoculated into 5-7 mL of liquid media in a slant tube. The bacterial inoculum is then shaken for 24 hours. After ...
Escherichia coli is a pathogenic bacterium that lives in the intestines of humans and animals and plays a role in maintaining a healthy digestive system. This bacterium is also found in water and food, as are non-pathogenic strains of E. coli. Pathogenic strains, such as enterotoxigenic E. coli (EPEC), produce shiga toxin, which is an indicator of water pollution and invades the epithelial cells of the small intestine. The presence of E. coli in aquatic environments indicates contamination by waste or animal feces carried in the water.Escherichia coli is a type of bacteria belonging to the coliform family that normally lives in human and animal feces, therefore it is also called fecal coliform. E. coli is a Gram-negative, rod-shaped, non-spore-forming bacterium. E. coli is commonly found in water as a source of microbial pollution. This bacterium forms rods in chains with a length of approximately 2 micrometers and a diameter of 0.5 micrometers. This bacterium has Bacillus forms include monobacilli, diplobacilli, or streptobacilli, and do not form spores or capsules. The volume of E. coli generally ranges from 0.6-0.7 m3. Inappropriate E. coli culture can result in long filaments, rarely capsules, and motile E. coli strains.
E. coli bacteria have round, convex, smooth colonies with distinct edges. E. coli is classified as an opportunistic bacterium commonly found in the human large intestine as normal flora. E. coli motility can be either motile or non-motile. This pathogenic bacterium thrives at temperatures between 8-46°C, but its optimal temperature is 37°C. This temperature range allows E. coli to grow well in the human digestive tract. E. coli is known as a bacteria that causes diarrhea and digestive tract disorders. Not all E. coli are dangerous, but only a small number cause disease if their growth is uncontrolled. E. coli is generally harmless and can benefit humans by playing a role in the production of vitamin K. The presence of E. coli as part of the intestinal flora actually prevents the growth of other potentially harmful bacteria in the intestine.
[08.01, 11/6/2026] Imma: G
[12.43, 11/6/2026] Imma: Bacterial classification of Escherichia coli according to Priyanti (2017) is as follows:
Domain: Bacteria
Kingdom: Eubacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
Genus: Escherichia
Species: Escherichia coli
Escherichia coli is a bacterium belonging to the coliform group and normally lives in human and animal feces, so it is also called fecal coliform. E. coli is a Gram-negative bacterium, rod-shaped and non-spore-forming. E. coli is commonly found in water as an indicator of microbial pollutant load. The bacterium has a rod/chain shape with a length range of 2 micrometers and a diameter of 0.5 micrometers. This bacterium has a bacillus form; some are monobacillus, diplobacillus, or streptobacillus. It does not form spores or capsules. The volume of E. coli generally ranges between 0.6-0.7 m³.
Improper cultivation of E. coli bacteria can result in long filamentous forms. Capsules are rarely present, and E. coli strains exhibit motility.
E. coli bacteria have round, convex, smooth colonies with distinct edges. E. coli is classified as an opportunistic bacterium commonly found in the human large intestine as normal flora. The motility possessed by E. coli bacteria can be motile or non-motile. This type of pathogenic bacterium grows well at temperatures of 8-46°C, but its optimal temperature is 37°C. Within that temperature range, E. coli can grow well in the human digestive tract. E. coli is known as a bacterium that causes diarrhea and digestive tract disorders. Not all E. coli are dangerous; only a small proportion cause disease if their growth is uncontrolled. In general, E. coli is harmless and can benefit humans by playing a role in producing vitamin K. The presence of E. coli as intestinal flora actually prevents the growth of other bacteria that may be dangerous if they grow in the gut.
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